New alternatives for the development of vaccine preparations: contributions to the knowledge on the interaction of recombinant protein viral antigens with nucleic acids
نویسندگان
چکیده
Currently, there are no effective tools to fight multiple pathogens due to the insufficient knowledge on their components and the limited arsenal of vaccine alternatives. In the present paper, combinations of recombinant viral antigens and nucleic acids are studied using electron microscopy, electrophoresis, nuclease digestion and sucrose or cesium chloride gradients. We describe new data on morphology, size, and the increased stability of protein particles composed of viral antigens in close association with nucleic acids. We demonstrate, for the first time, that particles of recombinant HCV viral core proteins are able to interact with plasmid molecules for DNA immunization, forming complexes of particles with increased density and size. Additionally, the increased immunogenicity of these recombinant viral protein-plasmid DNA mixtures for DNA immunization was evidenced when administered in animal models, compared to the individual components. In addition, we discuss the possible mechanisms behind the enhancement of the immune response, based on the experimental evidence of the interaction between mixture components and their properties. The use of recombinant viral proteins interacting with nucleic acids as the active principle, and simultaneously as an adjuvant or molecular vehicle for DNA vaccines, has important implications for the development of new effective strategies to prevent and treat diseases caused by different pathogens.
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